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As a dipeptide, aspartame is made of two normal, essential amino acids: phenylalanine (50 % by weight) and aspartic acid, (39 %), loosely bound together by a smaller methyl unit (11 %). At temperatures above body heat and high levels of acidity(low pH) after ingestion, it readily splits and releases its three components, which follow largely independent paths in humans.
As a dipeptide, aspartame is made of two normal, essential amino acids: phenylalanine (50 % by weight) and aspartic acid, (39 %), loosely bound together by a smaller methyl unit (11 %). At temperatures above body heat and high levels of acidity(low pH) after ingestion, it readily splits and releases its three components, which follow largely independent paths in humans.


==Safety and health effects==


===Controversy over safety and health effects===
====Methanol====
The methyl unit becomes methanol, better known as wood alcohol, CH3OH, which is usually an impurity in alcohol beverages, especially dark wines and liquors, at a level above 100 mg/l, one part in 10,000 by weight. Methanol itself is not very toxic, but in the body it is rapidly converted into formaldehyde (by the enzyme alcohol dehydrogenase) and thence largely into formic acid, with about 40 % of the methanol remaining as these two toxic reaction products.
However, the enzyme first is tied up with any available ethanol, C2H5OH, turning it into acetaldehyde, also toxic, until, after all the available ethanol is gone, the enzyme then quickly turns any methanol into formaldehyde.
Two teams published research in 1987 and 2005, showing that methanol in alcohol beverages taken before bedtime, resulted in hangover symptoms, especially headache, in healthy young students as long as 13 hours later, when the ethyl alcohol was all gone, but the remaining methanol was rapidly becoming formaldehyde.
The aspartame content of two liters diet soda, 5.6 12-oz cans, is 1,120 mg, releasing 11 % as 123 mg methanol.
Usually, there is not a concurrent larger amount of ethanol taken, which would prevent the production of formaldehyde.
So, the methanol from any aspartame is quickly turned into formaldehyde and formic acid.
An expert review by a competent, unbiased team led by M. Bouchard, 2001, cites references, many from aspartame industry funded studies, states that about 30 - 40 % of the methanol remains in the body as
unknown, durable reaction products.
J. Nutrition 1973 Oct; 103(10): 1454-1459.
Metabolism of aspartame in monkeys.<ref name="pmid4200872">{{cite journal |author=Oppermann JA, Muldoon E, Ranney RE |title=Metabolism of aspartame in monkeys |journal=J. Nutr. |volume=103 |issue=10 |pages=1454–9 |year=1973 |pmid=4200872 |url=http://jn.nutrition.org/cgi/reprint/103/10/1454}}</ref>
Oppermann JA, Muldoon E, Ranney RE.
Dept. of Biochemistry, Searle Laboratories,
Division of G.D. Searle and Co. Box 5110, Chicago, IL 60680
They found that about 70 % of the radioactive methanol in aspartame put into the stomachs of 3 to 7 kg monkeys was eliminated within 8 hours, with little additional elimination, as carbon dioxide in exhaled air and as water in the urine
They did not report any studies on the distribution of radioactivity in body tissues, except that blood plasma proteins after 4 days held 4 % of the initial methanol.
The low oral dose of aspartame and for methanol was 0.068 mmol/kg, about 1 part per million [ppm]
of the acute toxicity level of 2,000 mg/kg, 67,000 mmol/kg, used by McMartin (1979).
Two L daily use of diet soda provides 123 mg methanol, 2 mg/kg for a 60 kg person, a dose of 67 mmole/kg, a thousand times more than the dose in this study.
By eight hours excretion of the dose in air and urine had leveled off at 67.1 +-2.1 % as CO2 in the exhaled air and 1.57+-0.32 % in the urine, so 68.7 % was excreted, and 31.3 % was retained.
This data is the average of 4 monkeys.  "...the 14C in the feces was negligible."
"That fraction not so excreted (about 31%) was converted to body constituents through the one-carbon metabolic pool."  "All radioactivity measurements were counted to +-1 % accuracy..."
The abstract ends, "It was concluded that aspartame was digested to its three constituents that were then absorbed as natural constituents of the diet."
http://health.groups.yahoo.com/group/aspartameNM/message/1143
http://www.toxsci.oupjournals.org/cgi/content/full/64/2/169
"Exposure to methanol also results from the consumption of certain foodstuffs (fruits, fruit juices, certain vegetables, aspartame sweetener, roasted coffee, honey) and alcoholic beverages (Health Effects Institute, 1987; Jacobsen et al., 1988)."
"Experimental studies on the detailed time profiles following controlled repeated exposures to methanol are lacking."
"Thus, in monkeys and plausibly humans, a much larger fraction of body formaldehyde is rapidly converted to unobserved forms rather than passed on to formate and eventually CO2."
"However, the volume of distribution of formate was larger than that of methanol, which strongly suggests that formate distributes in body constituents other than water, such as proteins."
http://groups.yahoo.com/group/aspartameNM/message/1143
http://groups.yahoo.com/group/aspartameNM/message/1286
=== Methanol in alcohol abuse-related disorders ===
methanol products (formaldehyde and formic acid) are main cause of alcohol hangover symptoms [same as from similar amounts of methanol, the 11% part of aspartame]: YS Woo et al, 2005 Dec: Murray 2006.01.20
Addict Biol. 2005 Dec;10(4): 351-5.
Concentration changes of methanol in blood samples during an experimentally induced alcohol hangover state.
Woo YS, Yoon SJ, Lee HK, Lee CU, Chae JH, Lee CT, Kim DJ.
Chuncheon National Hospital, Department of Psychiatry, The Catholic University of Korea, Seoul, Korea. [ Han-Kyu Lee ]
A hangover is characterized by the unpleasant physical and mental symptoms that occur between 8 and 16 hours after drinking alcohol.
After inducing experimental hangover in normal individuals, we measured the methanol concentration prior to and after alcohol consumption and we assessed the association between the hangover condition
and the blood methanol level.
A total of 18 normal adult males participated in this study.
They did not have any previous histories of psychiatric or medical disorders.
The blood ethanol concentration prior to the alcohol intake (2.26+/-2.08) was not significantly different from that 13 hours after the alcohol consumption (3.12+/-2.38).
However, the difference of methanol concentration between the day of experiment (prior to the alcohol intake) and the next day (13 hours after the alcohol intake) was significant (2.62+/-1.33/l vs. 3.88+/-2.10/l, respectively).
[ So, the normal methanol level was 2.62 mg per liter, and increasing that by 50% = 1.3 mg per liter to 3.88 mg per liter caused hangover symptoms.
The human body has about 5.6 liters blood, so adding 1.3 mg per liter gives an estimate of 7.3 mg added methanol, as much as 4 oz diet soda.
Diet soda is about 200 mg aspartame per 12 oz can, which is 22 mg (11% methanol), 1.83 mg methanol per ounce.
Also, this 50 % increase in blood methanol that caused roughly similar symptoms in South Koreans, Woo YS, 2005, as in men in Swedem who had a 6-fold increase in urine methanol, confirms many studies that show that specific genetic differences make Asians and American Indians much more vulnerable to inebriation, hangover, and addiction than Europeans. Bendtsen P, Jones AW, Helander A. 1998 ]
A significant positive correlation was observed between the changes of blood methanol concentration
and hangover subjective scale score increment when covarying for the changes of blood ethanol level (r=0.498, p<0.05).
This result suggests the possible correlation of methanol as well as its toxic metabolite to hangover. PMID: 16318957
===The role of formaldehyde and formic acid===
[ The "toxic metabolite" of methanol is [[formaldehyde]], which in turn
partially becomes [[formic acid]] -- both potent cumulative toxins
that are the actual cause of the toxicity of methanol.]
<blockquote>Int J Neurosci. 2003 Apr; 113(4): 581-94.
The effects of alcohol hangover on cognitive functions in healthy subjects.<ref name="pmid12856484">{{cite journal |author=Kim DJ, Yoon SJ, Lee HP, Choi BM, Go HJ |title=The effects of alcohol hangover on cognitive functions in healthy subjects |journal=Int. J. Neurosci. |volume=113 |issue=4 |pages=581–94 |year=2003 |pmid=12856484 |doi=}}</ref>
Kim DJ, Yoon SJ, Lee HP, Choi BM, Go HJ.
Department of Psychiatry, College of Medicine, Catholic University of Korea, Buchon City, Kyunggi Do, Korea.
A hangover is characterized by the constellation of unpleasant physical and mental symptoms that occur between 8 and 16 h after drinking alcohol.
We evaluated the effects of experimentally-induced alcohol hangover on cognitive functions using the Luria-Nebraska Neuropsychological Battery.
A total of 13 normal adult males participated in this study.
They did not have any previous histories of psychiatric or medical disorders.
We defined the experimentally-induced hangover condition at 13 h after drinking a high dose of alcohol (1.5 g/kg of body weight).
We evaluated the changes of cognitive functions before drinking alcohol and during experimentally-induced hangover state.
The Luria-Nebraska Neuropsychological Battery was administrated in order to examine the changes of cognitive functions.
Cognitive functions, such as visual, memory, and intellectual process functions, were decreased during the hangover state.
Among summary scales, the profile elevation scale was also increased.
Among localization scales, the scores of left frontal, sensorimotor, parietal-occipital dysfunction, and right parietal-occipital scales were increased during the hangover state.
These results indicate that alcohol hangovers have a negative effect on cognitive functions, particularly on the higher cortical and visual functions associated with the left hemisphere and right posterior hemisphere.  Publication Types: Clinical Trial PMID: 12856484</blockquote>
<blockquote>Alcohol Alcohol. 1998 Jul-Aug; 33(4): 431-8.
Urinary excretion of methanol and 5-hydroxytryptophol as biochemical markers of recent drinking in the hangover state.<ref name="pmid9719404">{{cite journal |author=Bendtsen P, Jones AW, Helander A |title=Urinary excretion of methanol and 5-hydroxytryptophol as biochemical markers of recent drinking in the hangover state |journal=Alcohol Alcohol. |volume=33 |issue=4 |pages=431–8 |year=1998 |pmid=9719404 |doi=}}</ref> [email protected]
Bendtsen P, Jones AW, Helander A. [email protected]
Drug Dependence Unit, University Hospital, Linkoping, Sweden.
Twenty healthy social drinkers (9 women and 11 men) drank either 50 g of ethanol (mean intake 0.75 g/kg) or 80 g (mean 1.07 g/kg) according to choice as white wine or export beer in the evening over 2 h with a meal.
After the end of drinking, at bedtime, in the following morning after waking-up, and on two further occasions during the morning and early afternoon, breath-alcohol tests were performed and samples of urine were collected for analysis of ethanol and methanol and the 5-hydroxytryptophol (5-HTOL) to 5-hydroxyindol-3-ylacetic acid (5-HIAA) ratio.
The participants were also asked to quantify the intensity of hangover symptoms (headache, nausea, anxiety, drowsiness, fatigue, muscle aches, vertigo) on a scale from 0 (no symptoms) to 5 (severe symptoms).
The first morning urine void collected 6-11 h after bedtime as a rule contained measurable amounts of ethanol, being 0.09 +/- 0.03 g/l (mean +/- SD) after 50 g and 0.38 +/- 0.1 g/l after 80 g ethanol.
The corresponding breath-alcohol concentrations were zero, except for three individuals who registered 0.01-0.09g/l.
Ethanol was not measurable in urine samples collected later in the morning and early afternoon.
The peak urinary methanol occurred in the first morning void, when the mean concentration after 80 g ethanol was approximately 6-fold higher than pre-drinking values.</blockquote>
[ This is a much greater increase of methanol than the 50 % increase that cause roughly similar symptoms in South Koreans, Woo YS, 2005, confirming many studies that show that specific genetic differences make Asians and American Indians much more vulnerable to inebriation, hangover, and addiction. ]
This compares with a approximately 50-fold increase for the 5-HTOL/5-HIAA ratio in the first morning void.
Both methanol and the 5-HTOL/5-HIAA ratio remained elevated above pre-drinking baseline values in the second and sometimes even the third morning voids.
Most subjects experienced only mild hangover symptoms after drinking 50 g ethanol (mean score 2.4 +/- 2.6), but the scores were significantly higher after drinking 80 g (7.8 +/- 7.1).
The most common symptoms were headache, drowsiness, and fatigue.
A highly significant correlation (r = 0.62-0.75, P <0.01) was found between the presence of headache, nausea, and vertigo and the urinary methanol concentration in the first and second morning voids, whereas 5-HTOL/5-HIAA correlated with headache and nausea.
These results show that analysing urinary methanol and 5-HTOL furnishes a way to disclose recent drinking after alcohol has no longer been measurable by conventional breath-alcohol tests for at least 5-10 h.
The results also support the notion that methanol may be an important factor in the aetiology of hangover. PMID: 9719404
http://groups.yahoo.com/group/aspartameNM/message/1373
===Drug-metabolizing enzymes===
aspartame rat brain toxicity re cytochrome P450 enzymes, expecially CYP2E1, Vences-Mejia A, Espinosa-Aguirre JJ et al, 2006 Aug, Hum Exp Toxicol: relevant abstracts re formaldehyde from methanol in alcohol drinks: Murray 2006.09.29
[ Rich Murray notes:
As a medical layman, noting that all readers are laymen for any topic outside the bounds of their specific expertise, I found related abstracts that illucidate the role of cytochrome P450 enzymes, especially the one most affected by aspartame, CYP2E1, in brain toxicity processes involving ethanol and methanol, suggesting avenues of research for alcohol addiction and hangover, and the possibilies of aspartame liver and brain toxicity from its 11 % methanol component. ]
"A major finding in this study was that the daily consumption of ASP at the two doses considered leads to an increment in the concentration and activity of CYP2B1/2, CYP2E1 and CYP3A2 in rat cerebral and cerebellar microsomes....
The highest increment (up to 25-fold over controls) in a CYP-associated activity induced by ASP in brain was that of 4-NPH corresponding to CYP2E1.
The results mentioned above must be reproduced using a broad range of ASP concentrations in order to define the existence of a dose-related effect.
As far as we know, this is the first report regarding modulation of brain CYPs by the widely used
sweetener ASP.
Specific induction of brain CYPs could constitute a local regulatory mechanism of enzyme activity, thus influencing drug response;
for tissues exhibiting low regenerative capacity, such as the brain, such modulation would probably be of major toxicological significance....
It has already been said that once ASP enters the organism, it is rapidly metabolized by intestinal
esterases and dipeptidases to aspartic acid, phenylalanine and methanol, substances normally found in the diet and body. 37
One hour after ASP intake at a dose of 200 mg/kg body weight by rats, corresponding to the acceptable FDA daily intake for the sweetener after species correction, increased plasma and brain phenylalanine levels by 62 % and 192 % respectively. 6
With regard to methanol, it accounts for about 10 % of the ASP weight administered. 38
We can hypothesize that the exposure to methanol at the two regimens used in this study ( about 7.5 and 12.5 mg/kg from the doses of 75 and 125 mg/kg ) could induce xenobiotic-metabolizing enzymes in a
similar way to that of the chronic administration of ethanol. 39....
If methanol is the metabolite responsible for the induction of brain CYP2E1 seen in this work, the question of why the hepatic CYP2E1 was not altered remains.
Experiments with the three metabolites resulting from ASP metabolism are currently being undertaken in our laboratory in order to address this question.
In conclusion, data obtained demonstrated that a daily consumption of ASP at doses of 75 and 125 mg/kg body weight over 30 days provokes a substantial increment in CYP enzymes involved in endogenous and exogenous molecules metabolism in the CNS of the rat.
Biological consequences of this phenomenon should be investigated in view of the high number of humans exposed to this artificial sweetener and because of the recent data indicating the potential carcinogenic effects of this compound. 41"
"The aim of this study was to explore the effect of orally ingested ASP upon the expression and activity of CYP families involved in the CNS of rats.
The chosen ASP doses of 75 and 125 mg/kg body weight used in this study are within the limits of
human consumption after species factor correction.
Because rats metabolize ASP faster than humans, 31 dose comparisons between them have usually been
corrected by a factor of 5.
After correction, doses used here are below the FDA (50 mg/kg body weight) and Health and Welfare Canada (40 mg/kg body weight) acceptable daily intake for ASP. 32,33"
Hum Exp Toxicol. 2006 Aug; 25(8): 453-9.
The effect of aspartame on rat brain xenobiotic-metabolizing enzymes.
Vences-Mejia A 1,
Labra-Ruiz N 1,
Hernandez-Martinez N 1,
Dorado-Gonzalez V 1,
Gomez-Garduno J 1,
Perez-Lopez I 1,
Nosti-Palacios R 1,
Camacho Carranza R 2,
Espinosa-Aguirre JJ 2.
Laboratorio de Toxicologia Genetica,
1: Instituto Nacional de Pediatria, Insurgentes Sur, 3700-C, 04530 Mexico, DF Mexico.
2: Instituto de Investigaciones Biomédicas, UNAM, Apartado postal 70228, Ciudad Universitaria 04510 México, D.F., México
http://www.biomedicas.unam.mx/index.asp
*Correspondence: JJ Espinosa-Aguirre, Instituto de Investigaciones Biome´dicas, UNAM, Apartado postal 70228, Ciudad Universitaria 04510 Me´xico, D.F., Me´xico
Human & Experimental Toxicology (2006) 25(8): 453-459.
www.sagepublications.com c 2006 SAGE Publications 10.1191/0960327106het646oa
[ Dra. Araceli Vences M
Jefa de Laboratorio de Toxicologia Genetica, 6° P de Hospital Laboratorios, 10 84 09 00 Ext.1410 -1448  [email protected]
ISRAEL PÉREZ LÓPEZ,
JAVIER J. ESPINOSA AGUIRRE, [email protected]
http://www.biomedicas.unam.mx/investigacionFrame.asp?ID=MG ]
Abstract
This study demonstrates that chronic aspartame (ASP) consumption leads to an increase of phase I metabolizing enzymes (cytochrome P450 (CYP)) in rat brain.
Wistar rats were treated by gavage with ASP at daily doses of 75 and 125 mg/kg body weight for 30 days.
Cerebrum and cerebellum were used to obtain microsomal fractions to analyse activity and protein levels of seven cytochrome P450 enzymes.
Increases in activity were consistently found with the 75 mg/kg dose both in cerebrum and cerebellum for all seven enzymes, although not at the same levels:
CYP2E1-associated 4-nitrophenol hydroxylase (4-NPH) activity was increased 1.5-fold in cerebrum and 25-fold in cerebellum;
likewise, CYP2B1-associated penthoxyresorufin O-dealkylase (PROD) activity increased 2.9- and 1.7-fold respectively,
CYP2B2-associated benzyloxyresorufin O-dealkylase (BROD) 4.5- and 1.1-fold,
CYP3A-associated erythromycin N-demethylase (END) 1.4- and 3.3-fold,
CYP1A1-associated ethoxyresorufin O-deethylase (EROD) 5.5- and 2.8-fold,
and CYP1A2-associated methoxyresorufin O-demethylase (MROD) 3.7- and 1.3-fold.
Furthermore, the pattern of induction of CYP immunoreactive proteins by ASP paralleled that of 4-NHP-, PROD-, BROD-, END-, EROD- and MROD-related activities only in the cerebellum.
Conversely, no differences in CYP concentration and activity were detected in hepatic microsomes of treated animals with respect to the controls, suggesting a brain-specific response to ASP treatment.
PMID: 16937917 Aug 14 2006 08:07:58
Key words: aspartame; brain; cytochrome P450; enzyme induction
Introduction
Sweeteners are paid special attention among food additives, as their use enables a sharp reduction in sugar consumption and a significant decrease in caloric intake while maintaining the desirable palatability of foods and soft drinks.
Sweeteners are also of primary importance as part of nutritional guidance for diabetes, a disease with increasing incidence in developed countries. 1-3
Aspartame (L-asparthyl-L-phenylalanine methyl ester, ASP) is one of the most widely used artificial sweeteners; it is a high-intensity sweetener added to a large variety of foods, most commonly found in
low-calorie beverages, desserts and tabletop sweeteners added to tea or coffee.
It does not enter into the bloodstream intact, but is hydrolyzed in the intestine to form aspartate,
phenylalanine and methanol, which are then absorbed into the circulation, elevating their levels in plasma and in brain phenylalanine and tyrosine levels as well. 4-6
Aspartate is a highly excitatory neurotransmitter 7 and phenylalanine is a precursor of catecholamines in the brain; 8 increased levels of these molecules could change the basic activity level of the brain to an unhealthy, constantly stimulated state.
Short-term studies on ASP consumption and memory loss have been conducted in humans and rodents and no relationship was found. 9-11
On the other hand, chronic studies have implicated ASP consumption in learning and memory.
Consumption of 9% ASP in the diet for 13 weeks affected learning behaviour in male rats, 12 while ASP exposure of guinea pigs to 500 mg/kg during gestation disrupted odour-associative learning in pups. 13
Recently, Christian et al. reported that chronic ASP consumption lengthened the time it took rats to find the reward in a T-maze and increased the number of muscarinic receptors in specific brain areas. 14
[ 12 Potts WJ, Bloss JL, Nutting EF. Biological properties of aspartame:
I. Evaluation of central nervous system effects. J Environ Pathol Toxicol 1980; 3: 341=353.�
13 Dow-Edwards DL, Scribani LA, Riley EP.
Impaired performance on odor-aversion testing following prenatal aspartame exposure in the guinea pig.
Neurotoxicol Teratol 1989; 11: 413-416.
14 Christian B, McConnaughey K, Bethea E, Brantley S, Coffey A, Hammond L, et al.
Chronic aspartame affects T-maze performance, brain cholinergic receptors and Na�, K�-ATPase in rats. P
Pharmacol Biochem Behav 2004; 78: 121-127. ]
Despite numerous toxicological studies of ASP and its components, its effects on metabolic and detoxification enzyme systems have received little attention.
Metabolic enzymes are of special interest as changes in their function could lead to an increased susceptibility of the organisms to the harmful effects of a variety of contaminants found in the environment and in food products. 15,16
The presence of cytochrome P450 (CYP) in the central nervous system (CNS) opens the question of
whether metabolism in endothelial cells may regulate the penetration of the xenobiotics into the
brain compartment. 17,18
The role of CYP in brain includes such diverse functions as aromatization of androgens to oestrogens,
formation of catechols, and it may also participate in the metabolism of neurotransmitters and of xenobiotics. 17,19
Moreover, lipophilic xenobiotics can diffuse through the endothelial cells of the brain capillaries
and enter the neuronal cells.
Thus, in situ activation in the neuronal cell could have far-reaching consequences by causing irreversible disruption of the neuronal function.
The brain is the target not only for a number of toxic compounds but also for several psychoactive drugs.
The metabolism of drugs in the brain can lead to local pharmacological modulation at the site of action and can result in variable drug response. 17
The purpose of this work is to study the effect of orally administered ASP on the activity of CYP in
the CNS of the rat.
The characterization of brain specific CYP and its regulation and localization within the CNS is gaining importance for the understanding of the potential role of these enzymes in the pathogenesis of neurodegenerative disorders and in the psychopharmacological modulation of drugs acting on the CNS. 17
Free Radic Res. 1997 Oct; 27(4): 369-75.
Decreased antioxidant defense mechanisms in rat liver after methanol
intoxication.
Skrzydlewska E,
Farbiszewski R.
Department of Instrumental Analysis, Medical Academy, Poland.
The primary metabolic fate of methanol is oxidation to formaldehyde and then to formate by enzymes of the liver.
Cytochrome P-450 and a role for the hydroxyl radical have been implicated in this process.
The aim of the paper was to study the liver antioxidant defense system in methanol intoxication, in doses of 1.5, 3.0 and 6.0 g/kg b.w., after methanol administration to rats.
In liver homogenates, the activities of Cu,Zn-superoxide dismutase and catalase were significantly increased after 6 h following methanol ingestion in doses of 3.0 and 6.0 g/kg b.w. and persisted up to 2-5 days, accompanied by significant decrease of glutathione reductase and glutathione peroxidase activities.
The content of GSH was significantly decreased during 6 hours to 5 days.
The liver ascorbate level was significantly diminished, too, while MDA levels were considerably increased after 1.5, 3.0 and 6.0 g/kg b.w. methanol intoxication.
Changes due to methanol ingestion may indicate impaired antioxidant defense mechanisms in the liver tissue.  PMID: 9416465
Wien Klin Wochenschr. 1988 Apr 29; 100(9): 282-8.
[Methanol--an up-to-now neglected constituent of all alcoholic beverages. A new biochemical approach to the problem of chronic alcoholism] [Article in German]
Sprung R,
Bonte W,
Lesch OM.
Institut fur Rechtsmedizin, Universitat Gottingen.
Alcoholism is usually understood as ethanolism.
There is some evidence that its oxidation product acetaldehyde may condense with endogenous amines to form tetrahydroisoquinoline (TIQ) and - tetrahydro-beta-carboline (THBC) alkaloids which ultimately might be responsible for addiction.
In most animal experiments pure ethanol solutions were fed, but chronic alcoholics prefer normal alcoholic beverages, and it is widely ignored that all these beverages without exception also
contain methanol.
Its metabolite formaldehyde is a much more potent reaction partner for TIQ and THBC formation than
acetaldehyde.
As our findings in chronic alcoholics proved that these persons in contrast to healthy subjects are able to oxidize methanol despite high ethanol levels, there must be a continuous leakage of formaldehyde.
And it seems possible that methanol plays a more significant role in the pathophysiology and possibly the etiology of chronic alcoholism than ethanol.  PMID: 3291400
Wien Klin Wochenschr. 1991; 103(22): 684-9.
[Methanol metabolism in chronic alcoholism] [Article in German]
Soyka M,
Gilg T,
von Meyer L,
Ora I.
Psychiatrische Klinik, Universitat Munchen.
Serum methanol concentrations (SMC) exceeding 10 mg/l are highly suggestive of long-term alcohol intoxication and can be considered as marker for chronic alcohol abuse.
Endogenously formed or consumed methanol is almost exclusively metabolized by alcohol dehydrogenase.
As long as blood alcohol concentrations exceed 0.2-0.5 g/l methanol cannot be metabolized and accumulates.
In a prospective study on 78 patients admitted for alcohol detoxification, elevated SMC up to 78 mg/l were found, with a mean SMC of 29.4 mg/l.
No correlation was demonstrated between SMC and severity of the alcohol withdrawal syndrome.
Further clinical, forensic and biochemical aspects of methanol metabolism are discussed. PMID: 1776249
FORMIC ACID:
folic acid prevents neurotoxicity from formic acid, made by body from methanol impurity in alcohol drinks [ also 11 % of aspartame ], BM Bhushan, PL Carlen, DC Lehotay, AC Vandenbroucke, Y Adamchik, U. of Toronto, 2007 Dec., Alcoholism Cl. Exp. Res.: Murray 2007.11.27
http://rmforall.blogspot.com/2007_11_01_archive.htm
Wednesday, November 27, 2007
http://groups.yahoo.com/group/aspartameNM/message/1495
[ See also:
http://rmforall.blogspot.com/2007_11_01_archive.htm
Wednesday, November 28, 2007
http://groups.yahoo.com/group/aspartameNM/message/1496
explosion in numbers of children with serious food allergies has bewildered experts and parents, Helen Francombe, The Australian 2007.11.17: role of formic acid from methanol in liquors and aspartame, Murray 2007.11.28 ]


==References==
==References==
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{{reflist|2}}

Revision as of 12:54, 22 December 2007

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Aspartame is the common name for aspartyl-phenylalanine-1-methyl ester, the methyl ester of the dipeptide of the amino acids aspartic acid and phenylalanine. It is a non-saccharide artificial sweetener, very commonly used in diet drinks and other low-calorie foods, though it is not always suitable in baked goods, as it breaks down under high heat and loses much of its sweetness. Aspartame has about the same nutritional energy content as sugar, but as it is about 180 times as sweet as sugar, the energy content of foods and drinks sweetened with aspartame is significantly lower than those sweetened with sugar.

Aspartame was approved by the U.S. Food and Drug Administration (FDA) for dry foods in July, 1981, for carbonated beverages in July, 1983,[1] and for all food uses since 1996.

As a dipeptide, aspartame is made of two normal, essential amino acids: phenylalanine (50 % by weight) and aspartic acid, (39 %), loosely bound together by a smaller methyl unit (11 %). At temperatures above body heat and high levels of acidity(low pH) after ingestion, it readily splits and releases its three components, which follow largely independent paths in humans.


References

  1. ↑ United States General Accounting Office (June 1987). Food Additive Approval Process Followed for Aspartame (PDF).
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